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胶质母细胞瘤FGFR3-TACC3融合基因介导丙酮酸激酶M2入核促进DNA损伤修复基础研究

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胶质母细胞瘤FGFR3-TACC3融合基因介导丙酮酸激酶M2入核促进DNA损伤修复基础研究

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_wanfang_journals_xdsjjbzz202308025

胶质母细胞瘤FGFR3-TACC3融合基因介导丙酮酸激酶M2入核促进DNA损伤修复基础研究

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Full title

胶质母细胞瘤FGFR3-TACC3融合基因介导丙酮酸激酶M2入核促进DNA损伤修复基础研究

Author / Creator

任修德 , 李涛 , 范吉康 , 王希森 , 贾晓丹 and 杨学军

Publisher

300052 天津医科大学总医院神经外科%102218 北京清华长庚医院神经外科

Journal title

中国现代神经疾病杂志, 2023-08, Vol.23 (8), p.745-757

Language

Chinese

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Articles

Publication information

Publisher

300052 天津医科大学总医院神经外科%102218 北京清华长庚医院神经外科

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Scope and Contents

Contents

目的 探讨胶质母细胞瘤FGFR3-TACC3(F3-T3)融合基因介导丙酮酸激酶M2(PKM2)入核激活DNA损伤修复致替莫唑胺(TMZ)耐药的作用机制.方法 慢病毒转染构建稳定表达F3-T3融合基因和空载体的胶质母细胞瘤细胞系U87MG和U251MG,构建稳定表达F3-T3融合基因的胶质母细胞瘤裸鼠模型,小动物活体成像系统观察荷瘤鼠肿瘤荧光信号强度;采用生物信息学分析基因芯片转录组数据分析F3-T3融合基因的生物学功能,并分析肿瘤基因组学图谱计划(TCGA)数据库中胶质瘤患者生存期与PKM2基因表达的关系;瞬时转染小干扰RNA(siRNA)敲低PKM2基因表达;CCK-8细胞增殖实验观察经梯度浓度替莫唑胺处理后、转染siRNA后、替莫唑胺联合PKM2抑制剂Compound 3k处理后U87...

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Full title

胶质母细胞瘤FGFR3-TACC3融合基因介导丙酮酸激酶M2入核促进DNA损伤修复基础研究

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Record Identifier

TN_cdi_wanfang_journals_xdsjjbzz202308025

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_wanfang_journals_xdsjjbzz202308025

Other Identifiers

ISSN

1672-6731

DOI

10.3969/j.issn.1672-6731.2023.08.015

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