曲古抑素A下调Bcl-2水平经线粒体途径诱导耐顺铂人肺腺癌细胞凋亡
曲古抑素A下调Bcl-2水平经线粒体途径诱导耐顺铂人肺腺癌细胞凋亡
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410008,长沙,中南大学湘雅医院呼吸内科
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Contents
背景与目的以铂类为基础的联合化疗是非小细胞肺癌目前首选的治疗方案,然而由于铂类药物的毒副作用和耐药的发生,限制了铂类药物的广泛应用。曲古抑素A（Trichostatin A,TSA）是一种组蛋白去乙酰化酶（histone deacetylase,HDAC）抑制剂,能诱导肿瘤细胞生长抑制、分化、凋亡,并可以增强多种肿瘤细胞对铂类药物敏感性。本研究探讨TSA诱导耐顺铂人肺腺癌A549/CDDP细胞株凋亡的作用及机制。方法中性红法测定TSA对A549/CDDP细胞毒性,荧光显微镜观察细胞DNA的变化,流式细胞仪分析细胞周期及线粒体膜电位的变化,分别转染Bcl-2表达质粒过表达Bcl-2和利用siRNA干扰Bcl-2表达。Western blot分析凋亡相关蛋白的变化。结果TSA对A549/CDDP...
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Full title
曲古抑素A下调Bcl-2水平经线粒体途径诱导耐顺铂人肺腺癌细胞凋亡
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Record Identifier
TN_cdi_wanfang_journals_zgfazz200911003
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_wanfang_journals_zgfazz200911003
Other Identifiers
ISSN
1009-3419
E-ISSN
1999-6187
DOI
10.3779/j.issn.1009-3419.2009.11.03
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