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Books under fire : a hit list of banned and challenged children's books / Pat Scales for the Office for Intellectual Freedom.

Books under fire : a hit list of banned and challenged children's books / Pat R. Scales (for the Office for Intellectual Freedom).

Kibble Awards for Literature 2018 : entries submitted.

Sub-series 1: Angus & Robertson book reviews in bound volumes, 1894-1970

Phosphotyrosine (pY) enrichment is critical for expanding fundamental and clinical understanding of cellular signaling by mass spectrometry-based proteomics. However, current pY enrichment methods exhibit a high cost per sample and limited reproducibility due to expensive affinity reagents and manual processing. We present rapid-robotic phosphotyrosine proteomics (R2-pY), which uses a magnetic particle processor and pY superbinders or antibodies. R2-pY handles 96 samples in parallel, requires 2 days to go from cell lysate to mass spectrometry injections, and results in global proteomic, phosphoproteomic and tyrosine specific phosphoproteomic samples. We benchmark the method on HeLa cells stimulated with pervanadate and serum and report over 4000 unique pY sites from 1 mg of peptide input, strong reproducibility between replicates, and phosphopeptide enrichment efficiencies above 99%. R2-pY extends our previously reported R2-P2 proteomic and global phosphoproteomic sample preparation framework, opening the door to large-scale studies of pY signaling in concert with global proteome and phosphoproteome profiling.Competing Interest StatementThe authors have declared no competing interest.Footnotes* http://www.ebi.ac.uk/pride

bioRxiv, 2023-01

Automated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics

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Books under fire : a hit list of banned and challenged children's books / Pat Scales for the Office for Intellectual Freedom.

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Books under fire : a hit list of banned and challenged children's books / Pat R. Scales (for the Office for Intellectual Freedom).

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Kibble Awards for Literature 2018 : entries submitted.

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Sub-series 1: Angus & Robertson book reviews in bound volumes, 1894-1970

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Automated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics

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