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Item 04: Bookplates by Adrian Feint, 1922-1933

The authors use Ca
2+
imaging in freely behaving mice to look at the long-term dynamics of CA1 hippocampal place codes. They find that, in a familiar environment, there is substantial change in the population of place-coding cells over time, but the ensembles of these cells are sufficiently stable to preserve an accurate spatial representation across weeks.
Using Ca
2+
imaging in freely behaving mice that repeatedly explored a familiar environment, we tracked thousands of CA1 pyramidal cells' place fields over weeks. Place coding was dynamic, as each day the ensemble representation of this environment involved a unique subset of cells. However, cells in the ∼15–25% overlap between any two of these subsets retained the same place fields, which sufficed to preserve an accurate spatial representation across weeks.

Nature neuroscience, 2013-03, Vol.16 (3), p.264-266

Long-term dynamics of CA1 hippocampal place codes

A miniature epifluorescence microscope that can be carried by a freely-moving adult mouse allows cellular-level imaging of neuronal spiking or measurement of microcirculation during normal behavioral activities.
A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca
2+
dynamics.

Nature methods, 2008-11, Vol.5 (11), p.935-938

High-speed, miniaturized fluorescence microscopy in freely moving mice

An integrated, miniature (1.9 g) fluorescence microscope containing light source, optics and sensor allows high-speed, wide field of view imaging of calcium spiking in hundreds of neurons in freely moving mice. The mass-producible portable microscope is also useful for a variety of fluorescence assays for which size, cost and portability can be concerns.
The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal behavior. Here we introduce a miniature (1.9 g) integrated fluorescence microscope made from mass-producible parts, including a semiconductor light source and sensor. This device enables high-speed cellular imaging across ∼0.5 mm
2
areas in active mice. This capability allowed concurrent tracking of Ca
2+
spiking in >200 Purkinje neurons across nine cerebellar microzones. During mouse locomotion, individual microzones exhibited large-scale, synchronized Ca
2+
spiking. This is a mesoscopic neural dynamic missed by prior techniques for studying the brain at other length scales. Overall, the integrated microscope is a potentially transformative technology that permits distribution to many animals and enables diverse usages, such as portable diagnostics or microscope arrays for large-scale screens.

Nature methods, 2011-10, Vol.8 (10), p.871-878

Miniaturized integration of a fluorescence microscope

Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue. A challenge in the creation of such devices is the design and integration of miniaturized optical and mechanical components. Until recently, fiber-based fluorescence imaging was mainly limited to epifluorescence and scanning confocal modalities. Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging. An upcoming generation of fluorescence imaging devices will be based on microfabricated device components.

Nature methods, 2005-12, Vol.2 (12), p.941-950

Fiber-optic fluorescence imaging

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Item 04: Bookplates by Adrian Feint, 1922-1933

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Long-term dynamics of CA1 hippocampal place codes

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High-speed, miniaturized fluorescence microscopy in freely moving mice

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Miniaturized integration of a fluorescence microscope

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Fiber-optic fluorescence imaging

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