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Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_crossref_citationtrail_10_1073_pnas_1218620110

Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

About this item

Full title

Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

Publisher

United States: National Academy of Sciences

Journal title

Proceedings of the National Academy of Sciences - PNAS, 2013-03, Vol.110 (13), p.5004-5009

Language

English

Formats

Publication information

Publisher

United States: National Academy of Sciences

More information

Scope and Contents

Contents

Reported here is a piggyBac transposon-based expression system for the generation of doxycycline-inducible, stably transfected mammalian cell cultures for large-scale protein production. The system works with commonly used adherent and suspension-adapted mammalian cell lines and requires only a single transfection step. Moreover, the high uniform e...

Alternative Titles

Full title

Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_crossref_citationtrail_10_1073_pnas_1218620110

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_crossref_citationtrail_10_1073_pnas_1218620110

Other Identifiers

ISSN

0027-8424

E-ISSN

1091-6490

DOI

10.1073/pnas.1218620110

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