Spatiotemporal Dynamics of Guanosine 3′,5′-Cyclic Monophosphate Revealed by a Genetically Encoded, F...
Spatiotemporal Dynamics of Guanosine 3′,5′-Cyclic Monophosphate Revealed by a Genetically Encoded, Fluorescent Indicator
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United States: National Academy of Sciences
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Language
English
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United States: National Academy of Sciences
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To investigate the dynamics of guanosine 3′,5′-cyclic monophosphate (cGMP) in single living cells, we constructed genetically encoded, fluorescent cGMP indicators by bracketing cGMP-dependent protein kinase (cGPK), minus residues 1-77, between cyan and yellow mutants of green fluorescent protein. cGMP decreased fluorescence resonance energy transfe...
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Full title
Spatiotemporal Dynamics of Guanosine 3′,5′-Cyclic Monophosphate Revealed by a Genetically Encoded, Fluorescent Indicator
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TN_cdi_crossref_primary_10_1073_pnas_051631298
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_crossref_primary_10_1073_pnas_051631298
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ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.051631298
