Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium
Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium
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Publisher
England: BioMed Central Ltd
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Language
English
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Publisher
England: BioMed Central Ltd
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Major Clostridium difficile virulence factors are the exotoxins TcdA and TcdB. Due to the large size and poor stability of the proteins, the active recombinant TcdA and TcdB have been difficult to produce.
The toxin genes tcdA and tcdB were amplified by PCR using chromosomal DNA from a toxigenic strain as a template, and cloned into a shuttle ve...
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Full title
Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium
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TN_cdi_doaj_primary_oai_doaj_org_article_02ed7a6de555429cbed376bf9e460666
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_02ed7a6de555429cbed376bf9e460666
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ISSN
1471-2180
E-ISSN
1471-2180
DOI
10.1186/1471-2180-8-192
