A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluoroge...
A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid
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Publisher
Switzerland: MDPI AG
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Language
English
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Switzerland: MDPI AG
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Orotate phosphoribosyltransferase (OPRT) exists as a bifunctional enzyme, uridine 5'-monophosphate synthase, in mammalian cells and plays an important role in pyrimidine biosynthesis. Measuring OPRT activity has been considered important for understanding biological events and development of molecular-targeting drugs. In this study, we demonstrate...
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Full title
A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid
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TN_cdi_doaj_primary_oai_doaj_org_article_0783f91e1420433a846fb8da68796ab4
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_0783f91e1420433a846fb8da68796ab4
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ISSN
1424-8220
E-ISSN
1424-8220
DOI
10.3390/s23052507
