Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments
Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments
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England: BioMed Central Ltd
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English
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England: BioMed Central Ltd
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Chromatin immunoprecipitation (ChIP) is an antibody-based approach that is frequently utilized in chromatin biology and epigenetics. The challenge in experimental variability by unpredictable nature of usable input amounts from samples and undefined antibody titer in ChIP reaction still remains to be addressed. Here, we introduce a simple and quick...
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Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments
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TN_cdi_doaj_primary_oai_doaj_org_article_233f4c97b89d44f49f48f10054c79ef8
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_233f4c97b89d44f49f48f10054c79ef8
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ISSN
1471-2164
E-ISSN
1471-2164
DOI
10.1186/s12864-023-09253-0