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Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_233f4c97b89d44f49f48f10054c79ef8

Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

About this item

Full title

Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Publisher

England: BioMed Central Ltd

Journal title

BMC genomics, 2023-04, Vol.24 (1), p.171-171, Article 171

Language

English

Formats

Publication information

Publisher

England: BioMed Central Ltd

More information

Scope and Contents

Contents

Chromatin immunoprecipitation (ChIP) is an antibody-based approach that is frequently utilized in chromatin biology and epigenetics. The challenge in experimental variability by unpredictable nature of usable input amounts from samples and undefined antibody titer in ChIP reaction still remains to be addressed. Here, we introduce a simple and quick...

Alternative Titles

Full title

Titration-based normalization of antibody amount improves consistency of ChIP-seq experiments

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_233f4c97b89d44f49f48f10054c79ef8

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_233f4c97b89d44f49f48f10054c79ef8

Other Identifiers

ISSN

1471-2164

E-ISSN

1471-2164

DOI

10.1186/s12864-023-09253-0

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