Ultraplexing: increasing the efficiency of long-read sequencing for hybrid assembly with k-mer-based...
Ultraplexing: increasing the efficiency of long-read sequencing for hybrid assembly with k-mer-based multiplexing
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Publisher
England: BioMed Central Ltd
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English
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Publisher
England: BioMed Central Ltd
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Hybrid genome assembly has emerged as an important technique in bacterial genomics, but cost and labor requirements limit large-scale application. We present Ultraplexing, a method to improve per-sample sequencing cost and hands-on time of Nanopore sequencing for hybrid assembly by at least 50% compared to molecular barcoding while maintaining high...
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Ultraplexing: increasing the efficiency of long-read sequencing for hybrid assembly with k-mer-based multiplexing
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TN_cdi_doaj_primary_oai_doaj_org_article_27d2e90e2aef4c37946658d63f2bd900
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_27d2e90e2aef4c37946658d63f2bd900
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ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-020-01974-9
