Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Metal ions at the active site of an enzyme act as cofactors, and their dynamic fluctuations can potentially influence enzyme activity. Here, we use λ-exonuclease as a model enzyme with two Mg
2+
binding sites and probe activity at various concentrations of magnesium by single-molecule-FRET. We find that while Mg
A
2+
and Mg
B
2...
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Full title
Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
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TN_cdi_doaj_primary_oai_doaj_org_article_2a0749b91eb14221840220e83adac0c3
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_2a0749b91eb14221840220e83adac0c3
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-018-06750-9
