A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiting step in synthetic biology. Here, we report a gRNA-tRNA array for CRISPR-Cas9 (GTR-CRISPR) for multiplexed engineering of
Saccharomyces cerevisiae
. Using reported gRNAs shown to be effective, this system enables simultaneous disruption of 8...
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Full title
A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
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TN_cdi_doaj_primary_oai_doaj_org_article_376af7d923074f22a079f38323a6a87a
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_376af7d923074f22a079f38323a6a87a
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-019-09005-3
