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One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclu...

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclu...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_47d417a77feb4dacbd62c6470563d175

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

About this item

Full title

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

Publisher

London: BioMed Central Ltd

Journal title

BMC biotechnology, 2020-07, Vol.20 (1), p.1-37, Article 37

Language

English

Formats

Publication information

Publisher

London: BioMed Central Ltd

More information

Scope and Contents

Contents

Spider silk is a proteinaceous fiber with remarkable mechanical properties spun from spider silk proteins (spidroins). Engineering spidroins have been successfully produced in a variety of heterologous hosts and the most widely used expression system is Escherichia coli (E. coli). So far, recombinantly expressed spidroins often form insoluble inclu...

Alternative Titles

Full title

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_47d417a77feb4dacbd62c6470563d175

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_47d417a77feb4dacbd62c6470563d175

Other Identifiers

ISSN

1472-6750

E-ISSN

1472-6750

DOI

10.1186/s12896-020-00630-1

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