Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite product...
Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
The CRISPR-Cas9 system has frequently been used for genome editing in
Streptomyces
; however, cytotoxicity, caused by off-target cleavage, limits its application. In this study, we implement innovative modification to Cas9, strategically addressing challenges encountered during gene manipulation using Cas9 within strains possessing high GC co...
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Full title
Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production
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TN_cdi_doaj_primary_oai_doaj_org_article_4834d17b26db4ecda07483cfd789f301
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_4834d17b26db4ecda07483cfd789f301
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-025-56278-y
