ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
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England: BioMed Central Ltd
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Language
English
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England: BioMed Central Ltd
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Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are s...
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ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics
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TN_cdi_doaj_primary_oai_doaj_org_article_545233f391c049b29663104de0e9910a
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_545233f391c049b29663104de0e9910a
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ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-021-02302-5
