Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassiu...
Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy
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Author / Creator
Tian, Xuejiao , Lin, Tzu-Yang , Lin, Po-Ting , Tsai, Min-Ju , Chen, Hsin , Chen, Wen-Jie , Lee, Chia-Ming , Tu, Chiao-Hui , Hsu, Jui-Cheng , Hsieh, Tung-Han , Tung, Yi-Chung , Wang, Chien-Kai , Lin, Suewei , Chu, Li-An , Tseng, Fan-Gang , Hsueh, Yi-Ping , Lee, Chi-Hon , Chen, Peilin and Chen, Bi-Chang
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Taking advantage of the good mechanical strength of expanded
Drosophila
brains and to tackle their relatively large size that can complicate imaging, we apply potassium (poly)acrylate-based hydrogels for expansion microscopy (ExM), resulting in a 40x plus increased resolution of transgenic fluorescent proteins preserved by glutaraldehyde fixa...
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Full title
Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy
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Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_63f18951a94544908ea2cafbb9d60043
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_63f18951a94544908ea2cafbb9d60043
Other Identifiers
ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-024-55305-8
