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Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and D...

Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and D...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_6bd8e3c1748a4c7081ee96531335338f

Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

About this item

Full title

Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Publisher

London: Nature Publishing Group UK

Journal title

Nature communications, 2017-12, Vol.8 (1), p.2090-9, Article 2090

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC) hardware to overcome this limitation. We assay our achievab...

Alternative Titles

Full title

Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_6bd8e3c1748a4c7081ee96531335338f

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_6bd8e3c1748a4c7081ee96531335338f

Other Identifiers

ISSN

2041-1723

E-ISSN

2041-1723

DOI

10.1038/s41467-017-02028-8

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