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Design of typical genes for heterologous gene expression

Design of typical genes for heterologous gene expression

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_887d83c7ccac4a97b8b80af5ba2fb735

Design of typical genes for heterologous gene expression

About this item

Full title

Design of typical genes for heterologous gene expression

Publisher

London: Nature Publishing Group UK

Journal title

Scientific reports, 2022-06, Vol.12 (1), p.9625-9625, Article 9625

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Heterologous protein expression is an important method for analysing cellular functions of proteins, in genetic circuit engineering and in overexpressing proteins for biopharmaceutical applications and structural biology research. The degeneracy of the genetic code, which enables a single protein to be encoded by a multitude of synonymous gene sequences, plays an important role in regulating protein expression, but substantial uncertainty exists concerning the details of this phenomenon. Here we analyse the influence of a profiled codon usage adaptation approach on protein expression levels in the eukaryotic model organism
Saccharomyces cerevisiae
. We selected green fluorescent protein (GFP) and human α-synuclein (αSyn) as representatives for stable and intrinsically disordered proteins and representing a benchmark and a challenging test case. A new approach was implemented to design typical genes resembling the codon usage of any subset of endogenous genes. Using this approach, synthetic genes for GFP and αSyn were generated, heterologously expressed and evaluated in yeast. We demonstrate that GFP is expressed at high levels, and that the toxic αSyn can be adapted to endogenous, low-level expression. The new software is publicly available as a web-application for performing host-specific protein adaptations to a set of the most commonly used model organisms (
https://odysseus.motorprotein.de
)....

Alternative Titles

Full title

Design of typical genes for heterologous gene expression

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Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_887d83c7ccac4a97b8b80af5ba2fb735

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_887d83c7ccac4a97b8b80af5ba2fb735

Other Identifiers

ISSN

2045-2322

E-ISSN

2045-2322

DOI

10.1038/s41598-022-13089-1

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