Meta‐analysis of genome‐wide DNA methylation and integrative omics of age in human skeletal muscle
Meta‐analysis of genome‐wide DNA methylation and integrative omics of age in human skeletal muscle
About this item
Full title
Author / Creator
Voisin, Sarah , Jacques, Macsue , Landen, Shanie , Harvey, Nicholas R. , Haupt, Larisa M. , Griffiths, Lyn R. , Gancheva, Sofiya , Ouni, Meriem , Jähnert, Markus , Ashton, Kevin J. , Coffey, Vernon G. , Thompson, Jamie‐Lee M. , Doering, Thomas M. , Gabory, Anne , Junien, Claudine , Caiazzo, Robert , Verkindt, Hélène , Raverdy, Violetta , Pattou, François , Froguel, Philippe , Craig, Jeffrey M. , Blocquiaux, Sara , Thomis, Martine , Sharples, Adam P. , Schürmann, Annette , Roden, Michael , Horvath, Steve and Eynon, Nir
Publisher
Germany: John Wiley & Sons, Inc
Journal title
Language
English
Formats
Publication information
Publisher
Germany: John Wiley & Sons, Inc
Subjects
More information
Scope and Contents
Contents
Background
Knowledge of age‐related DNA methylation changes in skeletal muscle is limited, yet this tissue is severely affected by ageing in humans.
Methods
We conducted a large‐scale epigenome‐wide association study meta‐analysis of age in human skeletal muscle from 10 studies (total n = 908 muscle methylomes from men and women aged 18–89 years old). We explored the genomic context of age‐related DNA methylation changes in chromatin states, CpG islands, and transcription factor binding sites and performed gene set enrichment analysis. We then integrated the DNA methylation data with known transcriptomic and proteomic age‐related changes in skeletal muscle. Finally, we updated our recently developed muscle epigenetic clock (https://bioconductor.org/packages/release/bioc/html/MEAT.html).
Results
We identified 6710 differentially methylated regions at a stringent false discovery rate <0.005, spanning 6367 unique genes, many of which related to skeletal muscle structure and development. We found a strong increase in DNA methylation at Polycomb target genes and bivalent chromatin domains and a concomitant decrease in DNA...
Alternative Titles
Full title
Meta‐analysis of genome‐wide DNA methylation and integrative omics of age in human skeletal muscle
Authors, Artists and Contributors
Author / Creator
Jacques, Macsue
Landen, Shanie
Harvey, Nicholas R.
Haupt, Larisa M.
Griffiths, Lyn R.
Gancheva, Sofiya
Ouni, Meriem
Jähnert, Markus
Ashton, Kevin J.
Coffey, Vernon G.
Thompson, Jamie‐Lee M.
Doering, Thomas M.
Gabory, Anne
Junien, Claudine
Caiazzo, Robert
Verkindt, Hélène
Raverdy, Violetta
Pattou, François
Froguel, Philippe
Craig, Jeffrey M.
Blocquiaux, Sara
Thomis, Martine
Sharples, Adam P.
Schürmann, Annette
Roden, Michael
Horvath, Steve
Eynon, Nir
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_ce55a89fa672408eba78329582af1e29
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_ce55a89fa672408eba78329582af1e29
Other Identifiers
ISSN
2190-5991
E-ISSN
2190-6009
DOI
10.1002/jcsm.12741
