Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome
Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and enrich RNAs of interest without biochemical manipulation before sequencing. Adaptive sampling pe...
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Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome
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TN_cdi_doaj_primary_oai_doaj_org_article_d4416d7febf74a18959042c201ce9036
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_d4416d7febf74a18959042c201ce9036
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-023-44656-3
