Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression le...
Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Single-cell RNA sequencing (scRNA-seq) has revolutionized the study of the cellular landscape of organs. Most single-cell protocols require fresh material, which limits sample size per experiment, and consequently, introduces batch effects. This is especially true for samples acquired through complex medical procedures, such as intestinal mucosal b...
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Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels
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TN_cdi_doaj_primary_oai_doaj_org_article_ea1f79c0e77a4d50adbee714e2e2a5a2
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_ea1f79c0e77a4d50adbee714e2e2a5a2
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-022-13812-y
