Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization proce...
Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process
About this item
Full title
Author / Creator
Publisher
England: BioMed Central Ltd
Journal title
Language
English
Formats
Publication information
Publisher
England: BioMed Central Ltd
Subjects
More information
Scope and Contents
Contents
The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs). However, recent advancement of biotechnology has shown substantial recovery of bioactive protein from such insoluble IBs by solubilization and r...
Alternative Titles
Full title
Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_f12149f7a02141dc8499dac4317ec54a
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_f12149f7a02141dc8499dac4317ec54a
Other Identifiers
ISSN
1475-2859
E-ISSN
1475-2859
DOI
10.1186/s12934-019-1053-9
