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sup.1H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be co...

sup.1H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be co...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_gale_infotracacademiconefile_A570250182

sup.1H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be considered in cell culture experiments

About this item

Full title

sup.1H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be considered in cell culture experiments

Publisher

Springer

Journal title

Analytical and bioanalytical chemistry, 2019-02, Vol.411 (4), p.797

Language

English

Formats

Publication information

Publisher

Springer

More information

Scope and Contents

Contents

HEPES is commonly used in cell culture media as a buffering substance. Compared to the bicarbonate/CO.sub.2 buffer system, it does not require a CO.sub.2 atmosphere, thereby ensuring stable pH values during handling of cell culture media outside of an incubator. Due to its intrinsic charge, HEPES is considered not to be taken up by cells, which was...

Alternative Titles

Full title

sup.1H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be considered in cell culture experiments

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Primary Identifiers

Record Identifier

TN_cdi_gale_infotracacademiconefile_A570250182

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_gale_infotracacademiconefile_A570250182

Other Identifiers

ISSN

1618-2642

DOI

10.1007/s00216-018-1518-4

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