Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a l...
Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell
About this item
Full title
Author / Creator
Publisher
United States: National Academy of Sciences
Journal title
Language
English
Formats
Publication information
Publisher
United States: National Academy of Sciences
Subjects
More information
Scope and Contents
Contents
We demonstrate far-field optical imaging with subdiffraction resolution of the endoplasmic reticulum (ER) in the interior of a living mammalian cell. The diffraction barrier is overcome by applying stimulated emission depletion (STED) on a yellow fluorescent protein tag. Imaging individual structural elements of the ER revealed a focal plane (x, y)...
Alternative Titles
Full title
Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_jstor_primary_25464218
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_jstor_primary_25464218
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.0807705105
