An Efficient Strategy for Broad-Range Detection of Low Abundance Bacteria without DNA Decontaminatio...
An Efficient Strategy for Broad-Range Detection of Low Abundance Bacteria without DNA Decontamination of PCR Reagents
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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Scope and Contents
Contents
Bacterial DNA contamination in PCR reagents has been a long standing problem that hampers the adoption of broad-range PCR in clinical and applied microbiology, particularly in detection of low abundance bacteria. Although several DNA decontamination protocols have been reported, they all suffer from compromised PCR efficiency or detection limits. T...
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Full title
An Efficient Strategy for Broad-Range Detection of Low Abundance Bacteria without DNA Decontamination of PCR Reagents
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TN_cdi_plos_journals_1298332803
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1298332803
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0020303
