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Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for bre...

Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for bre...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1473341065

Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies

About this item

Full title

Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies

Publisher

United States: Public Library of Science

Journal title

PloS one, 2013-12, Vol.8 (12), p.e83718-e83718

Language

English

Formats

Publication information

Publisher

United States: Public Library of Science

More information

Scope and Contents

Contents

A prerequisite to accurate interpretation of RQ-PCR data is robust data normalization. A commonly used method is to compare the cycle threshold (CT) of target miRNAs with those of a stably expressed endogenous (EC) miRNA(s) from the same sample. Despite the large number of studies reporting miRNA expression patterns, comparatively few appropriate E...

Alternative Titles

Full title

Identification and validation of miRNAs as endogenous controls for RQ-PCR in blood specimens for breast cancer studies

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_plos_journals_1473341065

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1473341065

Other Identifiers

ISSN

1932-6203

E-ISSN

1932-6203

DOI

10.1371/journal.pone.0083718

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