Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Dir...
Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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Contents
Glucose sensitivity and pH and thermal stabilities of Trichoderma reesei Cel1A (Bgl II) were improved by site-directed mutagenesis of only two amino acid residues (L167W or P172L) at the entrance of the active site. The Cel1A mutant showed high glucose tolerance (50% of inhibitory concentration = 650 mM), glucose stimulation (2.0 fold at 50 mM gluc...
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Full title
Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis
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TN_cdi_plos_journals_1758478792
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1758478792
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0147301
