D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamic...
D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii
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Publisher
United States: Public Library of Science
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Language
English
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Publisher
United States: Public Library of Science
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Contents
A d-allulose 3-epimerase from Flavonifractor plautii was cloned and expressed in Escherichia coli and Corynebacterium glutamicum. The maximum activity of the enzyme purified from recombinant E. coli cells was observed at pH 7.0, 65°C, and 1 mM Co2+ with a half-life of 40 min at 65°C, Km of 162 mM, and kcat of 25280 1/s. For increased d-allulose pro...
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Full title
D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii
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TN_cdi_plos_journals_1807561434
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1807561434
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0160044
