Expression, purification, and contaminant detection for structural studies of Ralstonia metalliduran...
Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1
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United States: Public Library of Science
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English
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United States: Public Library of Science
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Single-particle electron cryo-microscopy (cryo-EM) has become a popular method for high-resolution study of the structural and functional properties of proteins. However, sufficient expression and purification of membrane proteins holds many challenges. We describe methods to overcome these obstacles using ClC-rm1, a prokaryotic chloride channel (C...
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Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1
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TN_cdi_plos_journals_1917693679
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1917693679
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0180163
