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A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rps...

A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rps...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1979819692

A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rpsL as a counter-selection marker

About this item

Full title

A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rpsL as a counter-selection marker

Publisher

United States: Public Library of Science

Journal title

PloS one, 2014-09, Vol.9 (9), p.e109258

Language

English

Formats

Publication information

Publisher

United States: Public Library of Science

More information

Scope and Contents

Contents

The streptomycin counter-selection system is a useful tool for constructing unmarked in-frame gene deletions, which is a fundamental approach to study bacteria and their pathogenicity at the molecular level. A prerequisite for this system is acquiring a streptomycin-resistant strain due to rpsL mutations, which encodes the ribosomal protein S12. Ho...

Alternative Titles

Full title

A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rpsL as a counter-selection marker

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_plos_journals_1979819692

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1979819692

Other Identifiers

ISSN

1932-6203

E-ISSN

1932-6203

DOI

10.1371/journal.pone.0109258

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