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Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and...

Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2024147012

Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers

About this item

Full title

Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers

Publisher

United States: Public Library of Science

Journal title

PloS one, 2018-04, Vol.13 (4), p.e0195755-e0195755

Language

English

Formats

Publication information

Publisher

United States: Public Library of Science

More information

Scope and Contents

Contents

Sodium dodecyl sulfate electrophoresis (SDS) is a protein separation technique widely used, for example, prior to immunoblotting. Samples are usually prepared in a buffer containing both high concentrations of reducers and high concentrations of SDS. This conjunction renders the samples incompatible with common protein assays. By chelating the SDS,...

Alternative Titles

Full title

Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_plos_journals_2024147012

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2024147012

Other Identifiers

ISSN

1932-6203

E-ISSN

1932-6203

DOI

10.1371/journal.pone.0195755

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