Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and...
Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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Sodium dodecyl sulfate electrophoresis (SDS) is a protein separation technique widely used, for example, prior to immunoblotting. Samples are usually prepared in a buffer containing both high concentrations of reducers and high concentrations of SDS. This conjunction renders the samples incompatible with common protein assays. By chelating the SDS,...
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Optimization of the cydex blue assay: A one-step colorimetric protein assay using cyclodextrins and compatible with detergents and reducers
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TN_cdi_plos_journals_2024147012
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2024147012
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0195755