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Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Asse...

Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Asse...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2765305385

Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Assessing reference database, variable region and pre-processing bias in classification of mock bacterial community samples

About this item

Full title

Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Assessing reference database, variable region and pre-processing bias in classification of mock bacterial community samples

Publisher

United States: Public Library of Science

Journal title

PloS one, 2023-01, Vol.18 (1), p.e0280293-e0280293

Language

English

Formats

Publication information

Publisher

United States: Public Library of Science

More information

Scope and Contents

Contents

Microbiome research relies on next-generation sequencing and on downstream data analysis workflows. Several manufacturers have introduced multi-amplicon kits for microbiome characterization, improving speciation, but present unique challenges for analysis. The goal of this methodology study was to develop two analysis pipelines specific to mixed-or...

Alternative Titles

Full title

Multi-amplicon microbiome data analysis pipelines for mixed orientation sequences using QIIME2: Assessing reference database, variable region and pre-processing bias in classification of mock bacterial community samples

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_plos_journals_2765305385

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2765305385

Other Identifiers

ISSN

1932-6203

E-ISSN

1932-6203

DOI

10.1371/journal.pone.0280293

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