Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phen...
Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Crop plants contain large amounts of secondary compounds that interfere with protein extraction and gel-based proteomic analysis. Thus, a protein extraction protocol that can be easily applied to various crop materials with minimal optimization is essential. Here we describe a universal protocol for total protein extraction involving trichloroaceti...
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Full title
Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis
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TN_cdi_proquest_journals_1477985885
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_1477985885
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2014.022
