Biochemical isolation of Argonaute protein complexes by Ago-APP
Biochemical isolation of Argonaute protein complexes by Ago-APP
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United States: National Academy of Sciences
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Language
English
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Publisher
United States: National Academy of Sciences
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Contents
During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of all Ago protein complexes expressed in diverse spe...
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Full title
Biochemical isolation of Argonaute protein complexes by Ago-APP
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TN_cdi_proquest_journals_1717557544
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_1717557544
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ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.1506116112
