Simultaneous site-directed mutagenesis of duplicated loci in soybean using a single guide RNA
Simultaneous site-directed mutagenesis of duplicated loci in soybean using a single guide RNA
About this item
Full title
Author / Creator
Publisher
Berlin/Heidelberg: Springer Berlin Heidelberg
Journal title
Language
English
Formats
Publication information
Publisher
Berlin/Heidelberg: Springer Berlin Heidelberg
Subjects
More information
Scope and Contents
Contents
Key message
Using a gRNA and
Agrobacterium
-mediated transformation, we performed simultaneous site-directed mutagenesis of two
GmPPD
loci in soybean. Mutations in
GmPPD
loci were confirmed in at least 33% of T
2
seeds.
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated endonuclease...
Alternative Titles
Full title
Simultaneous site-directed mutagenesis of duplicated loci in soybean using a single guide RNA
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_journals_1992796926
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_1992796926
Other Identifiers
ISSN
0721-7714
E-ISSN
1432-203X
DOI
10.1007/s00299-018-2251-3
