SRF-miRNA-143．KLF-4信号通路在高糖诱导的人腹膜间皮细胞表型转换中的作用
SRF-miRNA-143．KLF-4信号通路在高糖诱导的人腹膜间皮细胞表型转换中的作用
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Beijing: People's Military Medical Press
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Chinese
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Beijing: People's Military Medical Press
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目的探讨Kruppel样因子4（KLF-4）在高糖刺激诱导的人腹膜问皮细胞（HPMC）表型转换中的作用。方法以50mmol／L葡萄糖刺激HPMC72h后，分别采用Real-timePCR及Westernblotting检测KLF-4、上皮细胞钙黏蛋白（E-cadherin）、α平滑肌肌动蛋白（α-SMA）、结缔组织生长因子（CTGF）及miRNA-143的表达。以LV-KLF-4及inhibitor转染高糖刺激72h后的HPMC，shRNA-KLF-4及mimic转染正常的HPMC，采用Real-timePCR及Westernblotting检测KLF-4、E-cadherin、α-SMA、CTGF及miRNA-143的表达。结果高糖刺激HPMC72h后，Real-timePCR检测结果显示...
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SRF-miRNA-143．KLF-4信号通路在高糖诱导的人腹膜间皮细胞表型转换中的作用
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TN_cdi_proquest_journals_2001669544
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2001669544
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ISSN
0577-7402
DOI
10.11855/j.issn.0577-7402.2016.12.06
