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Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application t...

Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application t...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2110393097

Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopy

About this item

Full title

Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopy

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

Journal title

bioRxiv, 2018-09

Language

English

Formats

Publication information

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

More information

Scope and Contents

Contents

A protein complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, the fragments reconstitute a functional r...

Alternative Titles

Full title

Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopy

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_journals_2110393097

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2110393097

Other Identifiers

E-ISSN

2692-8205

DOI

10.1101/423566