Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations
Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations
About this item
Full title
Author / Creator
Fulco, Charles P , Nasser, Joseph , Jones, Thouis R , Munson, Glen , Bergman, Drew T , Subramanian, Vidya , Grossman, Sharon R , Rockwell Anyoha , Patwardhan, Tejal A , Nguyen, Tung H , Kane, Michael , Doughty, Benjamin , Perez, Elizabeth M , Durand, Neva C , Stamenova, Elena K , Erez Lieberman Aiden , Lander, Eric S and Engreitz, Jesse M
Publisher
Cold Spring Harbor: Cold Spring Harbor Laboratory Press
Journal title
Language
English
Formats
Publication information
Publisher
Cold Spring Harbor: Cold Spring Harbor Laboratory Press
Subjects
More information
Scope and Contents
Contents
Mammalian genomes harbor millions of noncoding elements called enhancers that quantitatively regulate gene expression, but it remains unclear which enhancers regulate which genes. Here we describe an experimental approach, based on CRISPR interference, RNA FISH, and flow cytometry (CRISPRi-FlowFISH), to perturb enhancers in the genome, and apply it...
Alternative Titles
Full title
Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations
Authors, Artists and Contributors
Author / Creator
Nasser, Joseph
Jones, Thouis R
Munson, Glen
Bergman, Drew T
Subramanian, Vidya
Grossman, Sharon R
Rockwell Anyoha
Patwardhan, Tejal A
Nguyen, Tung H
Kane, Michael
Doughty, Benjamin
Perez, Elizabeth M
Durand, Neva C
Stamenova, Elena K
Erez Lieberman Aiden
Lander, Eric S
Engreitz, Jesse M
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_journals_2171273700
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2171273700
Other Identifiers
E-ISSN
2692-8205
DOI
10.1101/529990
How to access this item
https://www.proquest.com/docview/2171273700?pq-origsite=primo&accountid=13902
