Time-resolved cryo-EM using Spotiton
Time-resolved cryo-EM using Spotiton
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Author / Creator
Dandey, Venkata P. , Budell, William C. , Wei, Hui , Bobe, Daija , Maruthi, Kashyap , Kopylov, Mykhailo , Eng, Edward T. , Kahn, Peter A. , Hinshaw, Jenny E. , Kundu, Nidhi , Nimigean, Crina M. , Fan, Chen , Sukomon, Nattakan , Darst, Seth A. , Saecker, Ruth M. , Chen, James , Malone, Brandon , Potter, Clinton S. and Carragher, Bridget
Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Scope and Contents
Contents
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid...
Alternative Titles
Full title
Time-resolved cryo-EM using Spotiton
Authors, Artists and Contributors
Author / Creator
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Record Identifier
TN_cdi_proquest_journals_2439112678
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2439112678
Other Identifiers
ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/s41592-020-0925-6
