Scipion is a modular image processing framework integrating several software packages under a unified interface while taking care of file formats and conversions. Here new developments and capabilities of the Scipion plugin for the Relion software are presented and illustrated with the image processing pipeline of published data. The user interfaces of Scipion and Relion are compared and the key differences highlighted, allowing this manuscript to be used as a guide for both new and experienced users of these software. Different streaming image processing options are also discussed demonstrating the flexibility of the Scipion framework. Synopsis An overview of the Scipion plugin for the Relion software is presented and various capabilities of image processing within Scipion framework are discussed. Competing Interest Statement The authors have declared no competing interest. Footnotes * https://github.com/scipion-em/scipion-em-relion...

Using Relion software within Scipion framework

10.1101/2020.12.06.399808