Differentially optimized cell-free buffer enables robust expression from unprotected linear DNA in e...
Differentially optimized cell-free buffer enables robust expression from unprotected linear DNA in exonuclease-deficient extracts
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Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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Language
English
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Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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Contents
The use of linear DNA templates in cell-free systems promises to accelerate the prototyping and engineering of synthetic gene circuits. A key challenge is that linear templates are rapidly degraded by exonucleases present in cell extracts. Current approaches tackle the problem by adding exonuclease inhibitors and DNA-binding proteins to protect the...
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Full title
Differentially optimized cell-free buffer enables robust expression from unprotected linear DNA in exonuclease-deficient extracts
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TN_cdi_proquest_journals_2604996817
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2604996817
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E-ISSN
2692-8205
DOI
10.1101/2021.09.07.459228
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https://www.proquest.com/docview/2604996817?pq-origsite=primo&accountid=13902
