The use of biological products based on beneficial microorganisms that produce siderophores, which reduce the concentration of available to pathogensiron ions, can be successfully used in agriculture. The aim of our study was the sequencingand functional annotation of the genome of Bacillus subtilis strain from the collection of BIOTROF Ltd to identify genes associated with the synthesis of siderophores. Nucleotide sequences were analyzed using a MiSeq instrument (Illumina, Inc., USA) together with a MiSeq Reagent Kit v3 (300-cycle) (Illumina, Inc., USA). Functional genome annotation was carried out using the PROKKA 1.12 (https://github.com/kbaseapps/ProkkaAnnotation http://vicbioinformatics.com/) and RAST 2.0 (https://rast.nmpdr.org) programs. The KEGG Pathway database was used to assess the pool of genes associated with antimicrobial activity and construct a metabolic map (