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Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better...

Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2763156512

Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two

About this item

Full title

Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

Journal title

bioRxiv, 2023-01

Language

English

Formats

Publication information

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

More information

Scope and Contents

Contents

Background. In plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus pyogenes (SpCas9) predominantly induce small insertions or deletions at target sites. This can be used for inactivation of protein-coding genes by frame shift mutations. However, in some cases, it may be advantageous to delete larger chromosomal segments. This...

Alternative Titles

Full title

Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_journals_2763156512

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2763156512

Other Identifiers

E-ISSN

2692-8205

DOI

10.1101/2023.01.10.523375