Sequential introduction of reprogramming factors reveals a time-sensitive requirement for individual...
Sequential introduction of reprogramming factors reveals a time-sensitive requirement for individual factors and a sequential EMT–MET mechanism for optimal reprogramming
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Author / Creator
Liu, Xiaopeng , Sun, Hao , Qi, Jing , Wang, Linli , He, Songwei , Liu, Jing , Feng, Chengqian , Chen, Chunlan , Li, Wen , Guo, Yunqian , Qin, Dajiang , Pan, Guangjin , Chen, Jiekai , Pei, Duanqing and Zheng, Hui
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Present practices for reprogramming somatic cells to induced pluripotent stem cells involve simultaneous introduction of reprogramming factors. Here we report that a sequential introduction protocol (
Oct4
–
Klf4
first, then
c-Myc
and finally
Sox2
) outperforms the simultaneous one. Surprisingly, the sequential protocol acti...
Alternative Titles
Full title
Sequential introduction of reprogramming factors reveals a time-sensitive requirement for individual factors and a sequential EMT–MET mechanism for optimal reprogramming
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Record Identifier
TN_cdi_proquest_miscellaneous_1381099687
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1381099687
Other Identifiers
ISSN
1465-7392
E-ISSN
1476-4679
DOI
10.1038/ncb2765
