In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to f...
In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to facilitate the isolation of rare monoclonal antibodies from human B cells
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
The authors of this Protocol describe a system wherein human antigen-specific antibody secreting plasmablasts are enriched
in vivo
in a mouse host. The enriched plasmablasts can then be sorted by flow cytometry for subsequent IgG expression.
The ability to rapidly generate large panels of antigen-specific human antibodies in a rodent would...
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Full title
In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to facilitate the isolation of rare monoclonal antibodies from human B cells
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Record Identifier
TN_cdi_proquest_miscellaneous_1540230527
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1540230527
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2014.104
