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Directed evolution of APEX2 for electron microscopy and proximity labeling

Directed evolution of APEX2 for electron microscopy and proximity labeling

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1641425998

Directed evolution of APEX2 for electron microscopy and proximity labeling

About this item

Full title

Directed evolution of APEX2 for electron microscopy and proximity labeling

Publisher

New York: Nature Publishing Group US

Journal title

Nature methods, 2015-01, Vol.12 (1), p.51-54

Language

English

Formats

Publication information

Publisher

New York: Nature Publishing Group US

More information

Scope and Contents

Contents

A genetically encoded peroxidase with improved sensitivity, APEX2, is reported for electron microscopy and proximity labeling at low expression levels.
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requir...

Alternative Titles

Full title

Directed evolution of APEX2 for electron microscopy and proximity labeling

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_1641425998

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1641425998

Other Identifiers

ISSN

1548-7091

E-ISSN

1548-7105

DOI

10.1038/nmeth.3179

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