Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiv...
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Off-target cleavage by CAS9 or TALEN genome editing tools is detected by integrase-defective lentiviral vectors.
The utility of CRISPR-Cas9 and TALENs for genome editing may be compromised by their off-target activity. We show that integrase-defective lentiviral vectors (IDLVs) can detect such off-target cleavage with a frequency as low as 1%. I...
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Full title
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
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TN_cdi_proquest_miscellaneous_1664203714
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1664203714
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ISSN
1087-0156
E-ISSN
1546-1696
DOI
10.1038/nbt.3127
