Construction of a human scFv antibody library with VH regions randomized and its application
Construction of a human scFv antibody library with VH regions randomized and its application
About this item
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Author / Creator
Publisher
Dordrecht: Springer-Verlag
Journal title
Language
English
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Publisher
Dordrecht: Springer-Verlag
Subjects
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Scope and Contents
Contents
A non-immunized human single chain variable fragment (scFv) library containing 2.5 × 107 individual clones was constructed from antibody variable region genes of 200 non-immunized donors. ScFv gene repertories were generated by randomly combining rearranged variable regions of heavy chain (VH) and natural occurring light chain (VL) using overlappin...
Alternative Titles
Full title
Construction of a human scFv antibody library with VH regions randomized and its application
Authors, Artists and Contributors
Author / Creator
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Record Identifier
TN_cdi_proquest_miscellaneous_1685798648
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1685798648
Other Identifiers
ISSN
0141-5492
E-ISSN
1573-6776
DOI
10.1007/s10529-012-0898-3
