CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice
CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice
About this item
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Author / Creator
Weber, Julia , Öllinger, Rupert , Friedrich, Mathias , Ehmer, Ursula , Barenboim, Maxim , Steiger, Katja , Heid, Irina , Mueller, Sebastian , Maresch, Roman , Engleitner, Thomas , Gross, Nina , Geumann, Ulf , Fu, Beiyuan , Segler, Angela , Yuan, Detian , Lange, Sebastian , Strong, Alexander , de la Rosa, Jorge , Esposito, Irene , Liu, Pentao , Cadiñanos, Juan , Vassiliou, George S. , Schmid, Roland M. , Schneider, Günter , Unger, Kristian , Yang, Fengtang , Braren, Rickmer , Heikenwälder, Mathias , Varela, Ignacio , Saur, Dieter , Bradley, Allan and Rad, Roland
Publisher
United States: National Academy of Sciences
Journal title
Language
English
Formats
Publication information
Publisher
United States: National Academy of Sciences
Subjects
More information
Scope and Contents
Contents
Here, we show CRISPR/Cas9-based targeted somatic multiplexmutagenesis and its application for high-throughput analysis of gene function in mice. Using hepatic single guide RNA (sgRNA) delivery, we targeted large gene sets to induce hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC). We observed Darwinian selection of target ge...
Alternative Titles
Full title
CRISPR/Cas9 somatic multiplex-mutagenesis for high-throughput functional cancer genomics in mice
Authors, Artists and Contributors
Author / Creator
Öllinger, Rupert
Friedrich, Mathias
Ehmer, Ursula
Barenboim, Maxim
Steiger, Katja
Heid, Irina
Mueller, Sebastian
Maresch, Roman
Engleitner, Thomas
Gross, Nina
Geumann, Ulf
Fu, Beiyuan
Segler, Angela
Yuan, Detian
Lange, Sebastian
Strong, Alexander
de la Rosa, Jorge
Esposito, Irene
Liu, Pentao
Cadiñanos, Juan
Vassiliou, George S.
Schmid, Roland M.
Schneider, Günter
Unger, Kristian
Yang, Fengtang
Braren, Rickmer
Heikenwälder, Mathias
Varela, Ignacio
Saur, Dieter
Bradley, Allan
Rad, Roland
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_1732601344
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1732601344
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.1512392112
