Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and...
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation
About this item
Full title
Author / Creator
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publication information
Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
This protocol describes the long-term culture of liver and pancreas 3D organoids from human and mouse, and differentiation of liver organoids
in vitro
and
in vivo
. Methodology for genetic manipulation of these self-renewing organoids is also detailed.
Adult somatic tissues have proven difficult to expand
in vitro
, largely bec...
Alternative Titles
Full title
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation
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Record Identifier
TN_cdi_proquest_miscellaneous_1814682342
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1814682342
Other Identifiers
ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2016.097