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Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and...

Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1814682342

Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation

About this item

Full title

Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2016-09, Vol.11 (9), p.1724-1743

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

This protocol describes the long-term culture of liver and pancreas 3D organoids from human and mouse, and differentiation of liver organoids
in vitro
and
in vivo
. Methodology for genetic manipulation of these self-renewing organoids is also detailed.
Adult somatic tissues have proven difficult to expand
in vitro
, largely bec...

Alternative Titles

Full title

Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_1814682342

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1814682342

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/nprot.2016.097

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